Hydrogen sulfide detection and zebrafish imaging by a designed sensitive and selective fluorescent probe based on resorufin.

Yahui Sun, Xiaojie Tang, Kaikai Zhang, Kelin Liu, Zhao Li, Liangliang Zhao

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2022 Jan 05

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A new long-wavelength fluorescent probe 1 that could specifically identify H2S has been successfully synthesized and applied for imaging H2S in zebrafish. Probe 1 was readily prepared by featuring nitrobenzene as the recognition unit coupled to resorufin. The fluorescence off-on response is based on the fact that H2S can reduce the nitro group to an amino group, followed by the 1,6-rearrangement-elimination and the release of resorufin. By evaluating the application abilities of probe 1 in vivo and vitro, it is shown that probe 1 has high sensitivity and selectivity to H2S, low background fluorescence interference, with a low detection limit of 17.30 μM. Notably, the occurrence of the reaction can be observed by the naked eye, and the color of the solution changes from yellow to pink. More importantly, it is the first time that using paper chips as carrier to detect H2S, which lays a foundation for the practical application of detecting H2S. The excellent analysis and application capabilities of probe 1 make it an effective tool for further application in practice. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Citation

Yahui Sun, Xiaojie Tang, Kaikai Zhang, Kelin Liu, Zhao Li, Liangliang Zhao. Hydrogen sulfide detection and zebrafish imaging by a designed sensitive and selective fluorescent probe based on resorufin. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 2022 Jan 05;264:120265