Francesca Rossi, Manuel Beltran, Michela Damizia, Chiara Grelloni, Alessio Colantoni, Adriano Setti, Gaia Di Timoteo, Dario Dattilo, Alvaro Centrón-Broco, Carmine Nicoletti, Maurizio Fanciulli, Patrizia Lavia, Irene Bozzoni
Molecular cell 2022 Jan 06Circular RNAs (circRNAs) are widely expressed in eukaryotes and are regulated in many biological processes. Although several studies indicate their activity as microRNA (miRNA) and protein sponges, little is known about their ability to directly control mRNA homeostasis. We show that the widely expressed circZNF609 directly interacts with several mRNAs and increases their stability and/or translation by favoring the recruitment of the RNA-binding protein ELAVL1. Particularly, the interaction with CKAP5 mRNA, which interestingly overlaps the back-splicing junction, enhances CKAP5 translation, regulating microtubule function in cancer cells and sustaining cell-cycle progression. Finally, we show that circZNF609 downregulation increases the sensitivity of several cancer cell lines to different microtubule-targeting chemotherapeutic drugs and that locked nucleic acid (LNA) protectors against the pairing region on circZNF609 phenocopy such effects. These data set an example of how the small effects tuned by circZNF609/CKAP5 mRNA interaction might have a potent output in tumor growth and drug response. Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.
Francesca Rossi, Manuel Beltran, Michela Damizia, Chiara Grelloni, Alessio Colantoni, Adriano Setti, Gaia Di Timoteo, Dario Dattilo, Alvaro Centrón-Broco, Carmine Nicoletti, Maurizio Fanciulli, Patrizia Lavia, Irene Bozzoni. Circular RNA ZNF609/CKAP5 mRNA interaction regulates microtubule dynamics and tumorigenicity. Molecular cell. 2022 Jan 06;82(1):75-89.e9
PMID: 34942120
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