Aconitine induces cell apoptosis via mitochondria and death receptor signaling pathways in hippocampus cell line.

Hui Wang, Yanbing Liu, Ziyu Guo, Kexin Wu, Yunhao Zhang, Yanan Tian, Baoyu Zhao, Hao Lu

Research in veterinary science 2022 Mar

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Aconitine is a plant toxin derived from aconitum genus and well known for its neurological and vascular toxicity. However, the mechanism of toxicity on the growth and apoptosis of the neurological cells has not been well investigated. In this study, we used HT22 cell lines derived from hippocampus to explore the mechanism. We began with examination of the viability and DA (dopamine) contents of cells treated with different dose of aconitine. In this study, we investigated the role of apoptosis in AC-induced HT22 cells. Our results showed that aconitine inhibited HT22 cells growth and increased DA contents in a dose dependent manner. Aconitine treatment induced apoptosis in HT22 cells and we found aconitine induced apoptosis by upregulating the expression of Bax, Cyto c, Apaf-1, Caspase9, Fas, Fas-L, Fadd, Caspase8, Caspase3 with concomitant decreasing of Bcl-2 and Bid expression. Collectively, results suggest that aconitine induce apoptosis through mitochondrial-mediated and death receptor signaling pathways in HT22 cells. Copyright © 2022 Elsevier Ltd. All rights reserved.

Citation

Hui Wang, Yanbing Liu, Ziyu Guo, Kexin Wu, Yunhao Zhang, Yanan Tian, Baoyu Zhao, Hao Lu. Aconitine induces cell apoptosis via mitochondria and death receptor signaling pathways in hippocampus cell line. Research in veterinary science. 2022 Mar;143:124-133