Correlation Engine 2.0
Clear Search sequence regions


  • gene (5)
  • genes myc (1)
  • human (2)
  • human cells (1)
  • mice (1)
  • MYC (7)
  • rna (1)
  • rna polymerase (1)
  • Sizes of these terms reflect their relevance to your search.

    The MYC oncogene has been studied for decades, yet there is still intense debate over how this transcription factor controls gene expression. Here, we seek to answer these questions with an in vivo readout of discrete events of gene expression in single cells. We engineered an optogenetic variant of MYC (Pi-MYC) and combined this tool with single-molecule RNA and protein imaging techniques to investigate the role of MYC in modulating transcriptional bursting and transcription factor binding dynamics in human cells. We find that the immediate consequence of MYC overexpression is an increase in the duration rather than in the frequency of bursts, a functional role that is different from the majority of human transcription factors. We further propose that the mechanism by which MYC exerts global effects on the active period of genes is by altering the binding dynamics of transcription factors involved in RNA polymerase II complex assembly and productive elongation. Copyright © 2022. Published by Elsevier Inc.

    Citation

    Simona Patange, David A Ball, Yihan Wan, Tatiana S Karpova, Michelle Girvan, David Levens, Daniel R Larson. MYC amplifies gene expression through global changes in transcription factor dynamics. Cell reports. 2022 Jan 25;38(4):110292

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 35081348

    View Full Text