Synthesis of globotriose-modified peptides for the preparation of a colorimetric biosensor to detect Shiga toxins.

Yun Kong, Fang Liu, Zhaoxi Liu, Jiayu Zhao, Qizheng Wu, Xunlian Zhang, Min Liu, Houcheng Zhang, Songqiang Liu, Xiaomei Zhang, Min Chen

Talanta 2022 Jun 01

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Globotriose (Gal-α1, 4-Gal-β1, 4-Glc) is involved in binding with Shiga toxins (Stxs) produced by Shigella dysenteriae and certain pathogenic Escherichia coli strains which could cause severe gastroenteritis and hemolytic uremic syndrome (HUS). Thus, this trisaccharide group and its derivatives provide potentials in the development of carbohydrate-based diagnostic and therapeutic reagents against bacterial infection. Instead of the tedious chemical synthesis of globotriose or its glycoconjugates, we reported a multi-step (step-wise) enzymatic synthesis system containing glucosyltransferase (ApNGT, E.C. 4.3.3.5), β-1, 4-galactosyltransferase (LgtB, E.C. 2.4.1.22) and α-1, 4-galactosyltransferase (LgtC, E.C. 2.4.1.44) to produce globotriose-containing glycopeptides. In addition, based on the specific binding between Stxs and globotriose, a cost-efficient, convenient, ultra-sensitive and specific colorimetric biosensor was further constructed to detect Stxs using glycoconjugated Au@Fe-TFPA-COP (globotriose@Au@Fe-TFPA-COP) as a nanoenzyme catalyst. We estimate that this method conveniently applied in the detection of Stx-producing bacteria and associated infectious diseases. Copyright © 2022 Elsevier B.V. All rights reserved.

Citation

Yun Kong, Fang Liu, Zhaoxi Liu, Jiayu Zhao, Qizheng Wu, Xunlian Zhang, Min Liu, Houcheng Zhang, Songqiang Liu, Xiaomei Zhang, Min Chen. Synthesis of globotriose-modified peptides for the preparation of a colorimetric biosensor to detect Shiga toxins. Talanta. 2022 Jun 01;243:123353