Derivatizing Nile Red fluorophores to quantify the heterogeneous polarity upon protein aggregation in the cell.

Rui Sun, Wang Wan, Wenhan Jin, Yulong Bai, Qiuxuan Xia, Mengdie Wang, Yanan Huang, Lianggang Zeng, Jialu Sun, Congcong Peng, Biao Jing, Yu Liu

Chemical communications (Cambridge, England) 2022 Apr 28

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Protein aggregation in the cell is often manifested by the formation of subcellular punctate structures. Herein, we modulated the solvatochromism and solubility of Nile Red fluorophore derivatives to quantitatively study the polarity inside pathogenic protein aggregates, revealing structure- and protein-dependent polarity heterogeneity.

Citation

Rui Sun, Wang Wan, Wenhan Jin, Yulong Bai, Qiuxuan Xia, Mengdie Wang, Yanan Huang, Lianggang Zeng, Jialu Sun, Congcong Peng, Biao Jing, Yu Liu. Derivatizing Nile Red fluorophores to quantify the heterogeneous polarity upon protein aggregation in the cell. Chemical communications (Cambridge, England). 2022 Apr 28;58(35):5407-5410