TPPU Downregulates Oxidative Stress Damage and Induces BDNF Expression in PC-12 Cells.

Qiong Wu, Minlin Lin, Peng Wu, Chongyan Zhao, Shuang Yang, Haiying Yu, Wenjiao Xian, Jingfang Song

Computational and mathematical methods in medicine 2022

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Ischemia-reperfusion is an ongoing clinical challenge that can lead to a series of pathological changes including oxidative stress. The inhibition of soluble epoxide hydrolase inhibitor (sEH) by 1-(1-propanoylpiperidin-4-yl)-3-[4-(trifluoromethoxy)phenyl]urea (TPPU) results in an anti-inflammatory, cardioprotective, and blood vessel growth-promoting effects. Therefore, this study focused on the protective effect of TPPU on a rat pheochromocytoma (PC-12) cell oxidative stress model induced by H2O2. CCK-8 and Hoechst 33342 were used to evaluate cell apoptosis and western blot to detect the apoptotic proteins and brain-derived neurotrophic factor (BDNF) expression. The incubation with 100 μM, 50 μM, and 25 μM TPPU significantly increased PC-12 cell viability. Epoxyeicosatrienoic acid (EET) pretreatment also protected PC-12 cells from oxidative stress. In addition, TPPU reduced caspase-3 and Bax expression and induced Bcl-2 expression, and EETs exerted the same effect on caspase-3 expression as TPPU. A positive relationship was found between TPPU or EET incubation and BDNF expression. These results revealed that TPPU reduced PC-12 cell oxidative stress injury induced by H2O2 and promoted BDNF expression. Copyright © 2022 Qiong Wu et al.

Citation

Qiong Wu, Minlin Lin, Peng Wu, Chongyan Zhao, Shuang Yang, Haiying Yu, Wenjiao Xian, Jingfang Song. TPPU Downregulates Oxidative Stress Damage and Induces BDNF Expression in PC-12 Cells. Computational and mathematical methods in medicine. 2022;2022:7083022