Schisandrin B protects boar spermatozoa against oxidative damage and increases their fertilization ability during in vitro storage.

Chuang Li, Hyun Ju Oh, Haixing Liu, Min Kyu Kim

Theriogenology 2023 Mar 01

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Oxidative stress due to low temperatures during in vitro preservation reduces boar spermatozoa quality. It has been proven that Schisandrin B (Sch-B) can act against oxidative stress in cells. Therefore, the purpose of this study was to investigate whether the treatment with Sch-B could improve the quality of boar sperm during storage at 17 °C. Semen samples were randomly divided into four groups and added to the Beltsville Thawing Solution containing different concentrations of Sch-B (0, 0.1, 0.5, and 1 mg/L) after collection. Each group was then preserved at 17 °C and the sperm motility, membrane integrity, and acrosome integrity were detected to determine the maximum available concentration of Sch-B for sperm. The optimal concentration was set at 0.1 mg/L and was used in subsequent experiments. Sperms treated with 0 and 0.1 mg/L Sch-B were evaluated for lipid peroxidation (MDA) and fertilization ability through in vitro fertilization. Finally, the quality of blastocysts which were formed by 0 and 0.1 mg/L Sch-B-treated sperm was determined. The results showed that compared with the control, the addition of 0.1 mg/L Sch-B improved boar sperm motility, and the addition of 0.1 and 0.5 mg/L Sch-B improved sperm membrane integrity and acrosome integrity. Treatment with 0.1 mg/L Sch-B reduced the level of MDA and increased the cleavage rate, blastocyst rate, and total cell number of blastocysts compared to the rate and number in the control group. However, no significant difference was observed in the ROS levels of blastocysts between the treatment and the control groups. The expression levels of CAT, SOD2, and Bcl-2 in IVF-blastocysts formed using sperm stored for one day at 17 °C were significantly higher than those in the control blastocysts. On day 4 of storage, CAT and Bcl-2 expression were significantly higher in IVF-blastocysts formed from sperm treated with 0.1 mg/L Sch-B than that in the control blastocysts. The ratio of Bax/Bcl-2 was also significantly higher in IVF-blastocysts formed using Sch-B-treated sperm. Our findings demonstrate that treatment with Sch-B can protect boar sperm from oxidative stress during liquid preservation and can increase the fertilization ability of the sperm. Copyright © 2022 Elsevier Inc. All rights reserved.

Citation

Chuang Li, Hyun Ju Oh, Haixing Liu, Min Kyu Kim. Schisandrin B protects boar spermatozoa against oxidative damage and increases their fertilization ability during in vitro storage. Theriogenology. 2023 Mar 01;198:194-201