Higher expression of Th1/Th2-related cytokines in the intestine of Wistar rats with ligature-induced periodontitis.

João Martins de Mello-Neto, Gayathiri Elangovan, Edilson Ervolino, Newell Walter Johnson, Anders Gustafsson, Carlos Marcelo da Silva Figueredo

Journal of periodontal research 2023 Jun

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This study aimed to investigate the impact of ligature-induced periodontitis (LIP) on histopathological and immunological outcomes in the colon of Wistar rats. It has been repeatedly shown that inflammatory bowel disease (IBD) patients are at higher risk of developing periodontitis and presenting worse oral health than non-IBD patients. However, whether the chronic inflammatory process around teeth contributes to the pathophysiology of IBD needs to be further explored. Thirteen Wistar rats were allocated into LIP (n = 7) and controls (n = 6). Half of the colon was processed for histopathological analyses and immunohistochemical (CD45); the other half was homogenized for immunological analyses. Periodontal destruction was confirmed by measuring the distance from the cementum-enamel junction to the mandible's apical position of the mesial interproximal bone. The immunological analyses were performed with the Bio-Plex Th1/Th2 assay. There was a significantly higher interproximal bone loss in LIP compared to controls. The LIP group showed a moderate infiltrate of inflammatory cells, predominantly mononucleated cells in the intestinal tissues. There was significantly higher expression of GM-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-10, IL-12 (p70), IL-13, and TNF-α in the intestinal tissues of LIP group compared to controls. Ligature-induced periodontitis was associated with an overexpression of Th1/Th2-related cytokines in the colon of Wistar rats. © 2023 The Authors. Journal of Periodontal Research published by John Wiley & Sons Ltd.

Citation

João Martins de Mello-Neto, Gayathiri Elangovan, Edilson Ervolino, Newell Walter Johnson, Anders Gustafsson, Carlos Marcelo da Silva Figueredo. Higher expression of Th1/Th2-related cytokines in the intestine of Wistar rats with ligature-induced periodontitis. Journal of periodontal research. 2023 Jun;58(3):588-595