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    This study investigated the effects of supplementation of low-temperature probiotics isolated from the intestines of olive flounder on the growth performance, digestibility, and regulation of intestinal microbiota and the expression of genes related to growth, immunity, and apoptosis in olive flounder. Bacteria showing high growth at approximately 15-20 °C, which is the temperature of olive flounder culture, were isolated and confirmed to be Pseudomonas species through 16S rRNA gene sequence analysis. Whole-genome sequencing revealed that the strain has a 6,195,122 bp single circular chromosome and a guanine-cytosine content of 59.9%. In the feeding trial, supplementation with 1 × 108 CFU/g of the isolate strain positively modulated growth performances, digestive enzyme activity, and gut microbiota composition of olive flounder. RT-qPCR for the comparison of growth, immunity, and apoptosis-related gene expression levels showed no significant differences between the groups. Therefore, the isolated host-associated low-temperature probiotics improved the growth performance of olive flounder by causing positive changes in digestive activity and intestinal microbial composition without affecting host gene expression. © 2024. The Author(s).

    Citation

    Su-Jeong Lee, Da-In Noh, Young-Sun Lee, Md Tawheed Hasan, Sang Woo Hur, Seunghan Lee, Seong-Mok Jeong, Jong Min Lee, Eun-Woo Lee, Kang-Woong Kim, Won Je Jang. Effects of host-associated low-temperature probiotics in olive flounder (Paralichthys olivaceus) aquaculture. Scientific reports. 2024 Jan 25;14(1):2134

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    PMID: 38273006

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