Hee Jeong Kong, Ji-Young Moon, Bo-Hye Nam, Young-Ok Kim, Woo-Jin Kim, Jeong-Ho Lee, Kyung-Kil Kim, Bong-Seok Kim, Sang-Yeob Yeo, Chang Hoon Lee, Kwang-Il Kang, Sang-Jun Lee
Biotechnology Research Division, National Fisheries Research and Development Institute, 408-1 Sirang-ri, Gijang-eup, Gijang-gun, Busan 619-705, Republic of Korea. konghj@nfrdi.go.kr
Fish & shellfish immunology 2011 AugAutophagy is an important cellular response to starvation and stress, and plays critical roles in embryogenesis, development, cell death, cancer, and immunity. Beclin-1 is one of the central regulators of autophagy in mammals. In the present study, we isolated a PoBeclin-1 cDNA from the olive flounder (Paralichthys olivaceus) by screening a flounder gill cDNA library and rapid amplification of cDNA ends (RACE). The PoBeclin-1 cDNA we isolated encodes a 447-amino acid polypeptide containing a conserved Bcl-2-binding domain. The deduced amino acid sequence of PoBeclin-1 showed high degrees of sequence identity (80.5-95.3%) with Beclin-1 from human, frog, mouse, zebrafish, and pufferfish. PoBeclin-1 transcripts were detected from 1 day post-hatching and were found to be ubiquitously expressed in the healthy flounder. Expression of PoBeclin-1 mRNA was increased in the kidney and spleen of flounders challenged with viral hemorrhagic septicemia virus (VHSV). When infected with VHSV, PoBeclin-1-overexpressing HINAE cells had low level (about 26%) of VHSV G transcripts compared to control cells. Taken together, these results suggest that PoBeclin-1 may play a role in the innate immune response to viral infection in the flounder. Copyright © 2011 Elsevier Ltd. All rights reserved.
Hee Jeong Kong, Ji-Young Moon, Bo-Hye Nam, Young-Ok Kim, Woo-Jin Kim, Jeong-Ho Lee, Kyung-Kil Kim, Bong-Seok Kim, Sang-Yeob Yeo, Chang Hoon Lee, Kwang-Il Kang, Sang-Jun Lee. Molecular characterization of the autophagy-related gene Beclin-1 from the olive flounder (Paralichthys olivaceus). Fish & shellfish immunology. 2011 Aug;31(2):189-95
PMID: 21605687
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