Bias in topoisomerase (TOPO)-cloning of multitemplate PCR products using locked nucleic acid (LNA)-substituted primers.

University of Bremen, Department of Microbe-Plant Interactions, CBIB (Center for Biomolecular Interactions Bremen), 28359 Bremen, Germany.

Journal of microbiological methods 2012 Dec

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Locked nucleic acid (LNA) modifications help to improve nucleic acid recognition in molecular biology applications. We report that LNA-substituted primers in PCR reactions may cause considerable cloning bias when the widely used topoisomerase-based ligation is used for cloning of multitemplate PCR products. Copyright © 2012 Elsevier B.V. All rights reserved.

Citation

Yuan Liu, Jessica Döring, Thomas Hurek. Bias in topoisomerase (TOPO)-cloning of multitemplate PCR products using locked nucleic acid (LNA)-substituted primers. Journal of microbiological methods. 2012 Dec;91(3):483-6