Inhibitory effect of cyclopamine on metastatic ability of EC109 cells and its mechanism].

To investigate the effect of cyclopamine on metastatic ability of human esophageal cancer EC109 cells and explore the possible mechanism. Transwell chamber assay and angiogenesis assay were used to examine the metastatic ability, invasiveness and angiogenesis of EC109 cells treated with cyclopamine for 48 h. The expression of Gli-1 mRNA was detected using RT-PCR, and Western blotting was used to examine the protein expressions of Gli-1, matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF). Inhibition of the hedgehog signaling pathway by cyclopamine suppressed the migration, invasion, and angiogenesis of EC109 cells. Cyclopamine treatment significantly lowered the expression of Gli-1 mRNA (P<0.05) and the protein expressions of Gli-1, MMP-9 and VEGF (P<0.05). Cyclopamine can significantly inhibit the metastatic capacity of EC109 cells possibly by down-regulating MMP-9 and VEGF expression as a result of Gli-1 inhibition.

Citation

Xiaoping Zuo, Zhiming Qin, Kaibin Wang, Xiangru Zheng, Liqian Chen. Inhibitory effect of cyclopamine on metastatic ability of EC109 cells and its mechanism]. Nan fang yi ke da xue xue bao = Journal of Southern Medical University. 2012 Dec;32(12):1828-32

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PMID: 23268421

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